Developed by Griptonite Games, Shinobi will also make use of StreetPass.
Shinobi is making his return on 3DS this September, Sega confirmed following a reveal on the cover of next month's Nintendo Power.
A prequel starring Jiro Musashi, the father of series star Joe Musashi, the game, simply titled Shinobi, will feature side-scrolling gameplay reminiscent of the original games. In addition, Shinobi will support StreetPass and offer a plethora of achievements and challenges.
The game, as previously rumored, is being developed by portable veterans Griptonite Games, best known for their work on the recent Metroid-esque Spider-Man titles on DS. Griptonite makes their 3DS debut in early June with the movie-based Green Lantern: Rise of the Manhunters.
Shinobi will be out in stores in September 2011.